Description

• General description: Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
• Application: Sephadex® G-50 has been used:to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension[1]in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography[2]in desalting the recombinant enzymes eluted in the fraction five and six[3]
• Features and Benefits: Desalts, removes contaminants, and transfers to a new buffer in one step.Suitable for DNA purification from small molecules using gel filtration.Features a small bead size, resulting in shorter diffusion distances.Considered a classic gel filtration resin.
• Other Notes: G5080-100G′s updated product number is GE17-0042-01G5080-500G′s updated product number is GE17-0042-02
• Legal Information: Sephadex is a registered trademark of Cytiva