MilliporeSigma™ Upstate™ SMRTα, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-100-57MI-Each-of-1 | In Stock | ₹ 55,714.89 |
17-100-57MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,714.89
GST (18%): ₹ 10,028.68
Total Price: ₹ 65,743.57
Antigen
SMRTα
Classification
Monoclonal
Formulation
Anti-SMRT (mouse monoclonal). One vial containing 50μL of unpurified mouse monoclonal ascites with 0.05% sodium azide. Negative Ascites (mouse). One vial containing 50μL of mouse ascites with 0.05% sodium azide. ChIP Primers, IκBα promoter. One vial containing 75μL of 5μM of each primer specific for human IĸBα promoter. FOR: GAC GAC CCC AAT TCA AAT CG; REV: TCA GGC TCG GGG AAT TTC C
Gene Symbols
NCOR2, CTG26, N-CoR2, SMAP270, SMRT, SMRTE, SMRTE-tau, TNRC14, TRAC, TRAC-1, TRAC1
Immunogen
Recombinant protein corresponding to human SMRT.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_001070729.1
Includes
The ChIPAb+ SMRT set includes the SMRT antibody, a negative control mouse ascites and qPCR primers which amplify a 299bp region of human IκBα promoter.
Content And Storage
-20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles
Isotype
IgG2a κ
Applications
ChIP Assay, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Gene Accession No.
Q9Y618
Host Species
Mouse
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes SMRT.
Target Species
Human, Mouse, Rat
Form
Ascites
Related Products
Description
- Specifically detects SMRTα Clone: in Human, Mouse, Rat samples, and it is validated for ChIP, Electromobility Shift Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blotting The Silencing mediator of retinoic acid & thyroid hormone receptor protein, commonly called the SMRT protein mediates the transcriptional repression of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription machinery
- Consistent with this activity, this protein is known to form a large corepressor complex containing SIN3A/B and histone deacetylases HDAC1 and HDAC2
- SMRT is also a component of the N-Cor repressor (nuclear receptor corepressor), a multi-subunit complex minimally composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2
- SMRT and nuclear receptor corepressor N-CoR are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.