MilliporeSigma™ Upstate™ RNA Pol IIα, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-620-MI-Each-of-1 | In Stock | ₹ 55,714.89 |
17-620-MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,714.89
GST (18%): ₹ 10,028.68
Total Price: ₹ 65,743.57
Antigen
RNA Pol IIα
Classification
Monoclonal
Formulation
Anti-RNA Pol II (mouse monoclonal IgG1,purified). One vial containing 25μg of purified antibody in 25μL volume. Normal Mouse IgG. One vial containing 25μg of mouse IgG in 25μL volume. Control Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human GAPDH promoter. FOR: TAC TAG CGG TTT TAC GGG CG; REV: TCG AAC AGG AGG AGC AGA GAG CGA
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes RNA Pol II
Target Species
Human, Mouse, Rat, S. cerevisiae, Yeast
Form
Purified
Applications
ChIP Assay, Immunofluorescence, Immunoprecipitation
Conjugate
Unconjugated
Gene Accession No.
P24928
Immunogen
The RNA Pol II antibody is made against a peptide corresponding to the C-terminal domain of RNA Pol II.
Quantity
25 Assays
Research Discipline
Epigenetics and Nuclear Function
Gene ID (Entrez)
NP_000928
Includes
RNA Pol II ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of RNA Pol II.
Content And Storage
−20°C for one year from date of shipment
Isotype
IgG1
Related Products
Description
- Specifically detects RNA Pol IIα Clone: in Human, Mouse, Rat, S
- cerevisiae, Yeast samples, and it is validated for ChIP, Immunofluorescence, Immunoprecipitation DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
- Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs
- Forms the polymerase active center together with the second largest subunit
- Pol II is the central component of the basal RNA polymerase II transcription machinery
- It is composed of mobile elements that move relative to each other
- RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template
- At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II
- A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition
- During transcription elongation, Pol II moves on the template as the transcript elongates
- Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing
- Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome