Novus Biologicals™ Apoptosis Detection Antibody Pack

Apoptosis Detection

Unconjugated

Mouse

3 Vials

NB100-56527 (Caspase-8 Antibody)-0.025mg, NB100-56599 (PARP Antibody)-0.025mg, NB100-56708 (Caspase-3 Antibody)-0.025mg

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, CyTOF-ready

The following antibodies are provided in Apoptosis Detection Antibody Bundle: 1. Caspase-3 (Pro and Active/Cleaved): 0.025mg: Localized to the large domain subunit: WB, IHC (paraffin), IHC (frozen): H, M, R: Western blot: pro and active forms seen 2. Caspase-8 (Pro and Active/Cleaved): 0.025mg: 370 PVETDSEEQP 379: WB: H, Ch, Rh: Western blot: pro and active forms seen 3. PARP (cleavage-specific): 0.025mg: A peptide containing amino acids near the 214/215-cleavage site: WB, Flow: Human: Western blot: specifically detects the cleaved form of PARP

The Apoptosis Detection Antbody Bundle contains monoclonal antibodies to three key apoptosis markers: caspase-3, caspase-8, and cleaved PARP. The caspase-3 and -8 antibodies both recognize pro and cleaved forms. The PARP antibody specifically recognizes the 89 kDa PARP fragment and does not recognized the full-length protein.

Human, Mouse, Rat