Armenian Hamster IgG Isotype Control (eBio299Arm), Brilliant Violet™ 480, eBioscience™, Invitrogen™

Description

• Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin
• Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes
• Use at the same concentration as the experimental antibody
• Brilliant Violet™ 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information
• Our internal testing suggests that Brilliant Violet™ 480 (BV480) is not compatible with methanol-based fixation
• Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser
• BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
• Powered by Sirigen.™ The isotype of a primary antibody and the application it is being used in can result in background staining
• Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence
• Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen
• While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts
• Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined
• If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.