NBP197275

Human IgG, Fc, Biotin, Isotype control, Novus Biologicals™

Manufacturer: Novus Biologicals

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Antigen

IgG Fc

Concentration

LYOPH

Dilution

Western Blot 1:1000, ELISA 1:5000 - 1:50000, Immunohistochemistry 1:500, Immunohistochemistry-Paraffin 1:500, Immunohistochemistry-Frozen, Dot Blot

Host Species

Human

Quantity

1 mg

Primary or Secondary

Primary

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent) to desired concentration.

Content And Storage

Store lyophilized antibody at 4°C in the dark. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles.

Applications

Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Dot Blot

Conjugate

Biotin

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free with 0.01% Sodium Azide

Purification Method

Ion exchange chromatography

Regulatory Status

RUO

Test Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Human IgG, anti-Human IgG F(c) and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab)2 or anti-Papain.

Target Species

Human

Isotype

IgG

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Description

  • IgG Fc specifically detects IgG Fc in Human samples
  • It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.