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13-977-782

Vinculin Monoclonal Antibody (7F9), Biotin, eBioscience™, Invitrogen™

Manufacturer: Invitrogen

Select a Size

Pack Size SKU Availability Price
Each of 1 13-977-782-Each-of-1 In Stock ₹ 27,412.00

13-977-782 - Each of 1

₹ 27,412.00

In Stock

Quantity

1

Base Price: ₹ 27,412.00

GST (18%): ₹ 4,934.16

Total Price: ₹ 32,346.16

Antigen

Vinculin

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P18206, P85972, Q64727

Gene Symbols

VCL

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

22330, 305679, 7414

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Clone

7F9

Conjugate

Biotin

Gene

VCL

Gene Alias

9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Human, Mouse, Rat

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin
  • Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions
  • Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression
  • Vinculin binds to talin, paxillin, and alpha-actinin
  • Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis
  • Applications Reported: This 7F9 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
  • Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 10 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Filtration: 0.2 μm post-manufacturing filtered
  • Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions
  • It functions as one of several interacting proteins involved in anchoring F-actin to the membrane
  • It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques
  • In the assembly of adhesion plaques, the beta subunit of integrin binds to talin
  • Talin binds to vinculin that interacts with alpha-actinin and possibly with itself
  • Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system
  • Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.