14-015-937
CD15 Monoclonal Antibody (HI98), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 14-015-937-Each-of-1 | In Stock | ₹ 1,22,108.00 |
14-015-937 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,22,108.00
GST (18%): ₹ 21,979.44
Total Price: ₹ 1,44,087.44
Antigen
CD15
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P22083
Gene Symbols
FUT4
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
2526
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Clone
HI98
Conjugate
Unconjugated
Gene
FUT4
Gene Alias
FUTIV, Lewis X, SSEA-1
Host Species
Mouse
Quantity
2 mg
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgM κ
Related Products
Description
- Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X
- This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes
- Monocytes express this structure at varying degree while lymphocytes are negative
- Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P)
- Applications Tested: The HI98 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- The HI98 antibody has also been tested by immunohistochemistry on FFPE tisue (IHC-P) at less than or equal to 10 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
- granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
- There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
- Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
- This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
- Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
- CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.