GP6 Monoclonal Antibody (HY101), eBioscience™, Invitrogen™

Description

• Description: The HY101 monoclonal antibody reacts with the glycoprotein VI (GPVI), a 62-kDa type-I transmembrane glycoprotein that is expressed on mature megakaryotes and platelets
• GPVI associates with the Fc receptor (FcR) gamma-chain to form a high affinity receptor for collagen
• GPVI has also been reported to bind convulxin (Cvx)
• Phosphorylation of the ITAMs present on the FcR gamma-chain leads to recruitment of Src family kinases and activation of downstream signal transduction
• GPVI has been reported to play a critical role in platelet activation and aggregation
• Applications Reported: This HY101 antibody has been reported for use in flow cytometric analysis, and western blotting
• Applications Tested: This HY101 antibody has been tested by flow cytometric analysis or immunoblotting
• In flow cytometry this can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets
• Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets
• Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels
• Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation
• In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss
• However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke.