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17-537-042

Amphiregulin Monoclonal Antibody (AREG559), APC, eBioscience™, Invitrogen™

Manufacturer: eBioscience

Select a Size

Pack Size SKU Availability Price
Each of 1 17-537-042-Each-of-1 In Stock ₹ 28,270.85

17-537-042 - Each of 1

₹ 28,270.85

In Stock

Quantity

1

Base Price: ₹ 28,270.85

GST (18%): ₹ 5,088.753

Total Price: ₹ 33,359.603

Antigen

Amphiregulin

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P15514

Gene Symbols

AREG

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

374

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

AREG559

Conjugate

APC

Gene

AREG

Gene Alias

amphiregulin; amphiregulin B; AR; AREG; AREGB; colorectum cell-derived growth factor; CRDGF; Mcub; MGC13647; schwannoma-derived growth factor; SDGF

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The AREG858 monoclonal antibody reacts with human Amphiregulin (AR)
  • AR is a member of the Epidermal Growth Factor (EGF) family and is expressed as a transmembrane precursor protein and released by proteolytic cleavage
  • Soluble AR is a ligand for the EGF receptor (EGFR) and the interaction promotes growth of normal epithelial cells, fibroblasts and keratinocytes
  • In the context of the immune system AR functions as an effector molecule for tissue repair and homeostasis, mediates resistance and tolerance to infection, and suppresses inflammation
  • Under different disease and activating conditions AR is expressed in T cells, group 2 innate lymphoid cells, basophils, mast cells, eosinophils, and neutrophils
  • Applications Reported: This AREG559 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This AREG559 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No
  • 88-8824) and protocol
  • Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
  • This may be used at 5 μL (0.5 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF
  • The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor
  • Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR
  • These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin
  • All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds
  • AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta
  • AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.