Phospho-SLP-76 (Tyr128) Monoclonal Antibody (HNDZ55), APC, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 17-903-742-Each-of-1 | In Stock | ₹ 35,333.00 |
17-903-742 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 35,333.00
GST (18%): ₹ 6,359.94
Total Price: ₹ 41,692.94
Antigen
Phospho-SLP-76 (Tyr128)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
Q13094
Gene Symbols
LCP2
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
3937
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
HNDZ55
Conjugate
APC
Gene
LCP2
Gene Alias
76 kDa tyrosine phosphoprotein; AI323664; BB161688; Lcp2; Lymphocyte cytosolic protein 2; lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa); lymphocyte cytosolic protein 2 (SH2 domain-containing leukocyte protein of 76kD); SH2 domain-containing leukocyte protein of 76 kDa; SH2 domain-containing leukocyte protein of 76kD; SLP76; SLP-76; SLP-76 tyrosine phosphoprotein; twm
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- This HNDZ55 MAb recognizes human SH2 domain-containing leukocyte protein of 76 kD (SLP-76) when phosphorylated on tyrosine 128 (Y128)
- SLP-76 is phosphorylated by ZAP-70 downstream of the T cell receptor (TCR)
- SLP-76 contains an acidic region that includes a PEST domain, several tyrosine residues that are phosphorylated following TCR ligation, a proline-rich domain, and an SH2 domain
- Numerous proteins associate with SLP-76 including Vav1, GADS, and PLC gamma 1, supporting the notion that SLP-76 functions as a critical adaptor protein downstream of the TCR
- SLP-76-deficient T cell lines or mice deficient in SLP-76 have shown that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function
- This antibody recognizes only human SLP-76 when phosphorylated on Y128
- It is not recommended for staining of mouse cells
- SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat
- The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice
- The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains
- The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation
- SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain
- A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein
- Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.