200006839

DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor™ Plus 647, Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

DYKDDDDK Tag

Concentration

1.0 mg/mL

Classification

Recombinant Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone, pH 6.5

Immunogen

Synthetic peptide (DYKDDDDK)

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Clone

8H8L17

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Conjugate

Alexa Fluor Plus 647

Host Species

Rabbit

Target Species

Tag

Gene Alias

DDDDK, DDDDK epitope tag, ECS epitope tag, ECS tag, FLAG, FLAG Tag

Isotype

IgG

Purification Method

Protein A

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Description

  • DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization
  • The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
  • It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies
  • The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies
  • The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended
  • FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.