ZO-1 Recombinant Mouse Monoclonal Antibody (ZO1-1A12), Invitrogen™
Mouse Recombinant Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
ZO-1
Concentration
1.0 mg/mL
Classification
Recombinant Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.4
Gene Alias
DKFZp686M05161; I79_005485; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); tight junction protein ZO-1; Tjp1; zo1; ZO-1; ZO-1 MDCK; zona occludens 1; zona occludens protein 1; zonula occludens 1; zonula occludens 1 protein; zonula occludens protein 1
Gene Symbols
TJP1
Isotype
IgG1 κ
Purification Method
Protein A/G
Gene
TJP1
Clone
ZO1-1A12
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Mouse
Gene Accession No.
P39447, Q07157
Gene ID (Entrez)
21872, 7082
Immunogen
Human recombinant ZO-1 fusion protein encompassing amino acids 334-634
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Description
- Recombinant mouse monoclonal antibodies are produced using in vitro expression systems
- Recombinant antibodies are produced using specific genes that code for the desired antibodies
- These genes are cloned into an expression vector and expressed in vitro
- The advantages of recombinant antibodies include better specificity and lot-to-lot consistency
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- ZO-1 forms complexes with either ZO-2 or ZO-3
- In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction
- ZO-1 expression is significantly reduced in many breast cancer lines
- ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue
- ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions
- ZO-1 may be involved in signal transduction at cell-cell junctions
- Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene
- Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.