GST Recombinant Mouse Monoclonal Antibody (GST 3-4C), Biotin, Invitrogen™
Mouse Recombinant Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
GST
Concentration
1.0 mg/mL
Classification
Recombinant Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.4
Immunogen
GST protein from Schistosoma japonicum expressed from the pGEX vector.
Primary or Secondary
Primary
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Clone
GST 3-4C
Applications
Western Blot
Conjugate
Biotin
Host Species
Mouse
Target Species
Tag
Gene Alias
activator protein 1; AP1; AP-1; cancer/testis antigen 48; c-Jun; CT48; deafness, X-linked 7; DFN7; enhancer-binding protein AP1; epididymis secretory protein Li 22; FAEES3; fatty acid ethyl ester synthase III; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; Glutathione S-transferase P; glutathione S-transferase pi 1; Glutathione-S-Transferase; gonad-specific transporter; GST; GST class-mu 1; GST class-pi; GST HB subunit 4; GST protein; GST Tag; GST1; GST3; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GSTP; GSTP1; GSTP1-1; GTH4; GTM1; H-B; HB subunit 4; HEL-S-22; JUN; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; MU; MU-1; OATP6A1; OATP-I; OATPY; Organic anion-transporting polypeptide 6A1; Organic anion-transporting polypeptide I; p39; PI; Proto-oncogene c-Jun; S-(hydroxyalkyl)glutathione lyase; SLC21A19
Isotype
IgG2b κ
Purification Method
Protein A/G
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Description
- GST Tag recombinant mouse monoclonal antibody conjugated with Biotin specifically recognizes GST Tag at both C and N-terminus of proteins
- Recombinant mouse monoclonal antibodies are produced using in vitro expression systems
- Recombinant antibodies are produced using specific genes that code for the desired antibodies
- These genes are cloned into an expression vector and expressed in vitro
- The advantages of recombinant antibodies include better specificity and lot-to-lot consistency
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions
- Glutathione Stransferase is used to create the GST gene fusion system
- This GST-fusion protein can then be purified from cells via its high affinity for glutathione
- Fusion Proteins offer an important biological assay for direct protein-to-protein interactions
- The GST gene fusion system has been introduced into numerous expression vectors.