CD3 Monoclonal Antibody (OKT3), NovaFluor™ Yellow 590, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD3
Concentration
5 μL/Test
Classification
Monoclonal
Host Species
Mouse
Target Species
Human
Gene Accession No.
P04234, P07766, P09693, P20963
Gene ID (Entrez)
915, 916, 917, 919
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Clone
OKT3
Applications
Flow Cytometry
Conjugate
NovaFluor Yellow 590
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
4930549J05Rik, A430104F18Rik, AI504783, antigen CD3D, delta polypeptide (TiT3 complex), antigen CD3E, epsilon polypeptide (TiT3 complex), antigen CD3G, gamma polypeptide, antigen CD3Z, zeta polypeptide, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, Cd247 molecule, CD3, CD3 antigen delta chain, CD3 antigen delta polypeptide, CD3 antigen gamma chain, CD3 antigen, delta polypeptide, CD3 antigen, delta subunit, CD3 antigen, epsilon polypeptide, CD3 antigen, gamma polypeptide, CD3 antigen, zeta polypeptide, CD3 delta, CD3 epsilon, CD3 epsilon chain, CD3 epsilon subunit, CD3 epsilon subunit precursor, CD3 gamma-chain, CD3 glycoprotein, CD3 glycoprotein precursor, CD3 molecule delta polypeptide, CD3 molecule, delta, CD3 molecule, epsilon, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma, CD3 molecule, gamma polypeptide, CD3 protein, CD3 TCR complex, CD3 type I transmembrane glycoprotein, CD3 type I transmembrane glycoprotein precursor, CD3 zeta chain, Cd3d, CD3D antigen d
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Gene
CD3g
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Description
- The OKT3 mAb reacts with an epitope on the epsilon-subunit within the human CD3 complex
- The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection
- The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex
- CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells
- Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation
- Antibody clones OKT3 and SK7 see different epitopes
- This OKT3 antibody has been reported for use in flow cytometric analysis
- This OKT3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 uL (0.2 ug) per test
- A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- NovaFluor dyes are not compatible with DNA intercalating viability dyes
- Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes
- Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer Excitation: 552 nm; Emission: 592 nm; Laser: 561 nm (Yellow) Laser CD3 Monoclonal antibody specifically detects CD3 in Human samples
- It is validated for Flow Cytometry