CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PE-Cyanine7, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 25-049-282-Each-of-1 | In Stock | ₹ 32,396.00 |
25-049-282 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 32,396.00
GST (18%): ₹ 5,831.28
Total Price: ₹ 38,227.28
Antigen
CD49d (Integrin alpha 4)
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide, pH 7.2
Gene Accession No.
Q00651
Gene Symbols
ITGA4
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16401
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
R1-2
Conjugate
PE-Cyanine7
Gene
ITGA4
Gene Alias
269C wild type, alpha 4 subunit of VLA-4 receptor, antigen CD49D, alpha-4 subunit of VLA-4 receptor, CD49 antigen-like family member D, CD49d, IA4, integrin alpha 4, integrin alpha-4, integrin alpha4B, Integrin alpha-IV, integrin subunit alpha 4, integrin, alpha 4, integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor), Itga4, Itga4B, ITGA-IV, LPAM subunit alpha, lymphocyte Peyer patch adhesion molecules subunit alpha, very late activation protein 4 receptor, alpha 4 subunit, VLA-4 receptor, alpha 4 subunit, VLA-4 subunit alpha
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit
- The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106)
- This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils
- CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1)
- Applications Reported: This R1-2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This may be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product No
- 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
- 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins
- Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains
- Alpha4 chain associates with either beta1 or beta7 chain
- It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain
- These ligands included Madcam, VCAM, and fibronectin
- Madcam is known as the principal ligand for integrin alpha4beta7
- Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.