25-599-980
MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), PE-Cyanine7, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
CAS Number: 28-14-8
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 25-599-980-Each-of-1 | In Stock | ₹ 15,664.00 |
25-599-980 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,664.00
GST (18%): ₹ 2,819.52
Total Price: ₹ 18,483.52
Antigen
MHC Class I (H-2Db)
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P01895, P01896, P01897, P01899, P01900, P14426, P14427
Gene Symbols
H2-D1
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
14964
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
28-14-8
Conjugate
PE-Cyanine7
Gene
H2-D1
Gene Alias
H-2 class I histocompatibility antigen, D-B alpha chain; H-2D; H2-D; H-2D cell surface glycoprotein; H-2D(B); H2D1; H2-D1; H2DB; H2-DC1-beta transplantation antigen protein; H2L; H2LD; histocompatibility 2, D region locus 1; MHC class I antigen; MHC class I H2 antigen; MHC class I H-2Dp; MHC H2-D-q alpha-chain; Q5k protein
Host Species
Mouse
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Other Options
| Image | Product Name | Manufacturer | Price Range | |
|---|---|---|---|---|
H-2Ld/H-2Db Mouse anti-Mouse, BB700, Clone: 28-14-8, BD OptiBuild™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, BUV661, Clone: 28-14-8, BD Biosciences™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, BV480, Clone: 28-14-8, BD OptiBuild™ | -- | ₹ 35,422.00 | ||
H2-Ld/H-2Db Mouse anti-Mouse, Oligo, Clone: 28-14-8, BD™ AbSeq ™ | -- | ₹ 35,600.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, BUV805, Clone: 28-14-8, BD Biosciences™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, Brilliant Violet 605, Clone: 28-14-8, BD Optibuild™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, Brilliant Violet 711, Clone: 28-14-8, BD Optibuild™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, BUV737, Clone: 28-14-8, BD Biosciences™ | -- | ₹ 35,422.00 | ||
H-2Ld/H-2Db Mouse anti-Mouse, RY586, Clone: 28-14-8, BD OptiBuild™ | -- | ₹ 24,920.00 | ||
MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), eBioscience™, Invitrogen™ | -- | -- |
...
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Description
- Description: The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q
- Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin
- Applications Reported: This 28-14-8 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 28-14-8 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This may be used at less than or equal to 1.0 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8
- HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.