25-999-842

IgM Monoclonal Antibody (SA-DA4), PE-Cyanine7, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 25-999-842-Each-of-1 In Stock ₹ 33,553.00

25-999-842 - Each of 1

₹ 33,553.00

In Stock

Quantity

1

Base Price: ₹ 33,553.00

GST (18%): ₹ 6,039.54

Total Price: ₹ 39,592.54

Antigen

IgM

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P01871

Gene Symbols

IGHM

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

3507

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

SA-DA4

Conjugate

PE-Cyanine7

Gene

IGHM

Gene Alias

AGM1; IGHM; immunoglobulin heavy constant mu; MU; VH

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

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Description

  • Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM
  • It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
  • Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific
  • This variation can be attributed to both donor variability and the anticoagulant used during blood draw
  • Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody
  • Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
  • This may be used at 5 μL (0.25 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
  • Please protect this vial and stained samples from light
  • Fixation: Samples can be stored in IC Fixation Buffer (Product No
  • 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.