CD14 Monoclonal Antibody (61D3), Brilliant Ultra Violet™ 496, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 36-401-4942-Each | In Stock | ₹ 31,118.85 |
36-401-4942 - Each
In Stock
Quantity
1
Base Price: ₹ 31,118.85
GST (18%): ₹ 5,601.393
Total Price: ₹ 36,720.243
Antigen
CD14
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P08571
Gene Symbols
Cd14
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
929
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
61D3
Conjugate
Brilliant Ultraviolet 496
Gene
Cd14
Gene Alias
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- The 61D3 mAb reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein
- CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells
- Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14
- Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 61D3 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5μL (1.0μg) per test Brilliant Ultra Violet 496 (BUV496) is a tandem dye that emits at 496 nm and is intended for use on cytometers equipped with an Ultraviolet (355 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Excitation: 350 nm; Emission: 496 nm; Laser: Ultraviolet Laser
- CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils
- CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form
- CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein
- The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles
- Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes
- Alternative splicing results in multiple transcript variants encoding the same CD14 isoform
- Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.