CD80 (B7-1) Monoclonal Antibody (2D10.4), Brilliant Ultra Violet™ 737, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 36-708-0941-Each-of-1 | In Stock | ₹ 17,889.00 |
36-708-0941 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,889.00
GST (18%): ₹ 3,220.02
Total Price: ₹ 21,109.02
Antigen
CD80 (B7-1)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P33681
Gene Symbols
CD80
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
941
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
2D10.4
Conjugate
Brilliant Ultraviolet 737
Gene
CD80
Gene Alias
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Host Species
Mouse
Quantity
25 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily
- CD80 is expressed by activated B cells, macrophages and dendritic cells
- In addition, activated T cells express this antigen
- CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
- The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
- Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 2D10.4 antibody has been pre-diluted and tested by flow cytometric analysis of Daudi cells
- This may be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Brilliant Ultraviolet 737 is a tandem dye that emits at 732 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser
- CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
- Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
- CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
- The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
- Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.