JNK/SAPK (pT183/pY185) Mouse, Unlabeled, Clone: 41, BD

Description

• The Ras signaling pathway links the signals from growth factor receptors with the activation of the MAPK kinase cascade of phosphorylation leading to cell growth and differentiation
• External stimuli, like endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression, ultimately dictating an adaptation to the new environment
• Small GTPases of the Rho family, including cdc42, Rac1, and Rho, transmit the stress signals that initiate the signal cascade
• JNK is a c-Jun kinase that was also identified as SAPK1 and MAPKp49
• JNK/SAPK, along with p38 and RK5/BMK1, comprise three classes of stress-activated MAPK groups
• Complete activation of JNK/SAPK requires the phosphorylation of both Thr183 and Tyr185, which are located in a Thr-X-Tyr motif
• The activation of these residues is believed to be carried out by MKK4 and MKK7
• Active JNK/SAPK phosphorylates other kinases and multiple transcription factors that induce expression of genes, such as proinflammatory cytokines
• Flow Cytometry, Western Blotting