FAK Monoclonal Antibody (ZF002), Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 39-650-0-Each-of-1 | In Stock | ₹ 44,500.00 |
39-650-0 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,500.00
GST (18%): ₹ 8,010.00
Total Price: ₹ 52,510.00
Antigen
FAK
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.1% sodium azide; pH 7.4
Gene Accession No.
O35346, P34152, Q05397
Gene Symbols
PTK2
Immunogen
Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG1 κ
Applications
ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Clone
ZF002
Conjugate
Unconjugated
Gene
PTK2
Gene Alias
CG10023; CG10023-PA; CG10023-PB; CG10023-PC; CG10023-PD; CG10023-PF; CG10023-PG; CT28129; DFAK; DFak56; Dmel\CG10023; Dmel_CG10023; DmFAK; EC 2.7.10.2; Fadk; FADK 1; Fak; Fak1; Fak56; fak56D; FAK65D; Fak-PA; Fak-PB; Fak-PC; Fak-PD; Fak-PF; Fak-PG; FAK-related non-kinase polypeptide; focal adhesion kinase; Focal adhesion kinase 1; focal adhesion kinase homolog; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase pp125FAK; focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; I79_019430; Kiaa4203; mKIAA4203; p125FAK; p41/p43FRNK; pFAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; protein tyrosine kinase 2; protein tyrosine kinase 2 L homeolog; protein-tyrosine kinase; protein-tyrosine kinase 2; PTK2; PTK2 protein tyrosine kinase 2; ptk2.L; tyrosine kinase; XELAEV_18032408mg; XPFAK
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
14083, 25614, 5747
Content And Storage
-20°C
Form
Liquid
Related Products
Description
- Mouse and rat FAK-1 proteins share 93% homology with human
- On western blots, this antibody identifies the target band at ∽110 kDa
- Reactivity has been confirmed with HeLa cell lysates and mouse and rat brain homogenates by western blotting
- Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling
- FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle
- FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors
- Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin
- Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase
- In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin
- In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis
- Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.