CD45 Monoclonal Antibody (HI30), Brilliant Violet™ 480, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 41-404-5942-Each-of-1 | In Stock | ₹ 31,328.00 |
41-404-5942 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,328.00
GST (18%): ₹ 5,639.04
Total Price: ₹ 36,967.04
Antigen
CD45
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P08575
Gene Symbols
PTPRC
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
5788
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
HI30
Conjugate
Brilliant Violet 480
Gene
PTPRC
Gene Alias
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA)
- CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets
- The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
- Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This HI30 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.125 μg) per test Brilliant Violet 480 (BV480) is a dye that emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions
- Our internal testing suggests that Brilliant Violet 480 (BV480) is not compatible with methanol-based fixation
- Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser
- BRILLIANT Violet is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license
- Powered by Sirigen
- CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
- CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues
- CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
- CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
- CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
- CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
- CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
- CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.