CD127 Monoclonal Antibody (A7R34), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 46-127-182-Each-of-1 | In Stock | ₹ 10,324.00 |
46-127-182 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 10,324.00
GST (18%): ₹ 1,858.32
Total Price: ₹ 12,182.32
Antigen
CD127
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide, pH 7.2
Gene Accession No.
P16872
Gene Symbols
Il7r
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16197
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
A7R34
Conjugate
PerCP-eFluor 710
Gene
Il7r
Gene Alias
CD127, CD127 antigen, CDW127, IL 7 receptor, IL 7R a, IL 7R subunit alpha, IL 7R a, IL 7Ra, IL 7Ra, IL7 receptor, IL-7 receptor alpha chain, IL7 receptor subunit alpha, IL-7 receptor subunit alpha, IL7R, IL-7R, IL7R a, IL7R alpha, IL7R subunit alpha, IL-7R subunit alpha, IL7R a, IL7RA, IL-7RA, IL-7Ralpha, IL-7R-alpha, IL7Ra, ILRA, interleukin 7 receptor, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, Interleukin7 receptor subunit alpha, interleukin-7 receptor subunit alpha, MGC107557, sCD127, soluble IL 7 receptor, soluble IL 7R
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
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Description
- Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor
- IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes
- In the periphery, mature T cells express CD127 at low level
- A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation
- Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127
- Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This may be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
- We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
- CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis
- The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer
- CD127 consists of an alpha chain and a gamma chain
- The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules
- CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells
- The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor
- Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.