4718368

Human TNF-alpha Membrane Form Fluorescein-conjugated Antibody, R&D Systems™

Mouse Monoclonal Antibody has been used in 4 publications

Manufacturer: Fischer Scientific

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Antigen

TNF-alpha

Dilution

Flow Cytometry 10 uL/10^6 cells

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide

Gene Alias

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Gene Symbols

TNF

Isotype

IgG1

Purification Method

Protein A or G purified from ascites

Test Specificity

Detects cell surface expressed TNF-alpha (membrane and receptor bound forms) by flow cytometry. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Clone

6401

Applications

Flow Cytometry

Conjugate

Fluorescein

Host Species

Mouse

Target Species

Human

Gene Accession No.

P01375

Gene ID (Entrez)

7124

Immunogen

E. coli-derived recombinant human TNF-alpha Gly57-Leu233 (predicted) Accession # P01375

Primary or Secondary

Primary

Content And Storage

Protect from light.Do not freeze.12 months from date of receipt, 2 to 8 degreesC as supplied.

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Description

  • TNF-alpha Monoclonal specifically detects TNF-alpha in Human samples
  • It is validated for Flow Cytometry.