His Tag Antibody (AD1.1.10) - Non-Recombinant Monoclonal, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
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Antigen
His Tag
Concentration
1 mg/mL
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Frozen)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cellular Markers, Epitope Tags
Immunogen
PAX6 transcription factor linked to histidine tag
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
AD1.1.10
Dilution
Western Blot 1:1000, Simple Western 1:250, Flow Cytometry 1:10 - 1:50, ELISA 1:100 - 1:500, Immunohistochemistry 1:100 - 1:300, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:300, In vitro assay
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Gene Alias
6 His epitope tag, 6X His, 6X-His, H, HHHHHH epitope tag, HHHHHH tag, HIS, His tag, Poly-histidine
Isotype
IgG1
Purification Method
Protein G purified
Test Specificity
This antibody is reactive to histidine tags.
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Description
- His Tag Monoclonal specifically detects His Tag in Epitope Tag samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry-Frozen, In vitro assay.