4982040

HIF-1 alpha Antibody (ESEE122), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 81 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

HIF-1 alpha

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators

Gene Accession No.

Q16665

Gene ID (Entrez)

3091

Immunogen

This HIF-1 alpha Antibody (ESEE122) was developed against Human HIF-1 alpha, corresponding to amino acids 329 - 530 [Uniprot# Q16665].

Primary or Secondary

Primary

Content And Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

93 kDa

Clone

ESEE122

Dilution

Western Blot 1:500-1:1000, Simple Western 1:2000, Flow Cytometry reported in scientific literature (Gestier S. et al), Immunohistochemistry 1:100-1:5000, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 26757928 Fig1G), Immunohistochemistry-Paraffin 1:100-1:5000, Immunohistochemistry-Frozen 1:100-1:5000, Immunoblotting

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Bovine, Canine

Gene Alias

AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp

Gene Symbols

HIF1A

Isotype

IgG1

Purification Method

Protein G purified

Test Specificity

Stains HIF-1 alpha in free-floating canine brain sections, according to customer reports.

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Description

  • HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting.