4990512

SSEA-1 Antibody (MC-480), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 7 publications

Manufacturer: Fischer Scientific

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Antigen

SSEA-1

Concentration

1 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cellular Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Immunology, Pancreatic Stem Cell Markers, Stem Cell Markers, Stem Cells

Formulation

PBS with 0.02% Sodium Azide

Gene ID (Entrez)

2526

Immunogen

F9 tetracarcinoma stem cells (X-irradiated).

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Clone

MC-480

Dilution

Western Blot 2 ug/ml, Simple Western 1:10, Flow Cytometry 2.5 ug/million cells, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Chimpanzee

Gene Alias

alpha-(1,3)-fucosyltransferase, CD15, EC 2.4.1, EC 2.4.1.-, EC 2.4.1.65, ELAM-1 ligand fucosyltransferase, ELFT, FCT3AELAM ligand fucosyltransferase, Fucosyltransferase 4, fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific), Fucosyltransferase IV, fucT-IV, FUC-TIV, FUTIV, Galactoside 3-L-fucosyltransferase, Lewis X, LeX, SSEA-1, stage-specific embryonic antigen 1

Gene Symbols

FUT4

Isotype

IgM

Purification Method

IgM purified

Test Specificity

Human and mouse. This antibody recognizes SSEA1 in mouse EC and ES cells but not human, but SSEA1 is expressed by some human differentiated cell types (ie: trophoblasts). Reactivity with Rat is reported by Kim et al. 2007 (PMID 17548530).

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Description

  • SSEA-1 Monoclonal specifically detects SSEA-1 in Human, Mouse, Rat, Chimpanzee samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.