CD300e (IREM-2) Monoclonal Antibody (UP-H2), PE, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-107-45-Each-of-1 | In Stock | ₹ 33,909.00 |
50-107-45 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,909.00
GST (18%): ₹ 6,103.62
Total Price: ₹ 40,012.62
Antigen
CD300e (IREM-2)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
Q496F6
Gene Symbols
CD300E
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
342510
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
UP-H2
Conjugate
PE
Gene
CD300E
Gene Alias
BC034097; CD300 antigen like family member E; CD300 antigen-like family member E; CD300e; CD300e antigen; CD300e molecule; Cd300le; CLM2; CLM-2; CMRF35A5; CMRF35-A5; CMRF35-like molecule 2; CMRF-35-like molecule-1; Immune receptor expressed on myeloid cells 2; IREM2; IREM-2; PIgR2; PIgR-2; poly-Ig receptor 2; polymeric immunoglobulin receptor 2; TREM5
Host Species
Mouse
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG1
Related Products
Description
- Description: The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE
- It is a member of the CMRF-35 family
- This 34 kDa Ig-like containing receptor is an activating receptor and through it's charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12
- Expression is found exclusively on monocytes and myeloid DC's
- Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs
- It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2
- In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes
- Applications Reported: This UP-H2 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- CD300e is a member of the glycoprotein family of cell surface proteins expressed on myeloid cells
- CD300e interacts with the TYRO protein tyrosine kinase-binding protein and is thought to be involved as an activating receptor
- CD300e is expressed on mature myeloid dendritic cells and monocytes, and is down regulated on immature dendritic cells
- Further, CD300e interacts with DAP-12, which mediates activating signals
- The alloreactive response of naïve T cells has been reported to become enhanced by the activation of CD300e
- Diseases associated with CD300e dysfunction include tungiasis and lice infestation.