Vinculin Monoclonal Antibody (7F9), eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-2863-Each-of-1 | In Stock | ₹ 15,931.00 |
50-112-2863 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,931.00
GST (18%): ₹ 2,867.58
Total Price: ₹ 18,798.58
Antigen
Vinculin
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P18206, P85972, Q64727
Gene Symbols
VCL
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
22330, 305679, 7414
Content And Storage
4° C
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Clone
7F9
Conjugate
Unconjugated
Gene
VCL
Gene Alias
9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
Host Species
Mouse
Quantity
25 μg
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin
- Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions
- Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression
- Vinculin binds to talin, paxillin, and alpha-actinin
- Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis
- Applications Reported: This 7F9 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections and formalin-fixed paraffin embedded tissue sections, immunocytochemistry, and microscopy
- Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on methanol-fixed or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 μg/mL
- This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval buffer and can be used at less than or equal to 5 μg/mL
- The antibody has been tested by western blot on reduced and non-reduced cell lysates and can be used at less than or equal to 5 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions
- It functions as one of several interacting proteins involved in anchoring F-actin to the membrane
- It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques
- In the assembly of adhesion plaques, the beta subunit of integrin binds to talin
- Talin binds to vinculin that interacts with alpha-actinin and possibly with itself
- Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system
- Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.