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50-112-3009

Ly-6G Monoclonal Antibody (1A8-Ly6g), Functional Grade, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-3009-Each-of-1 In Stock ₹ 16,999.00

50-112-3009 - Each of 1

₹ 16,999.00

In Stock

Quantity

1

Base Price: ₹ 16,999.00

GST (18%): ₹ 3,059.82

Total Price: ₹ 20,058.82

Antigen

Ly-6G

Classification

Monoclonal

Concentration

1 mg/mL

Formulation

PBS with no preservative; pH 7.2

Gene Accession No.

P35461

Gene Symbols

Ly6g

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

546644

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry, Functional Assay

Clone

1A8-Ly6g

Conjugate

Functional Grade

Gene

Ly6g

Gene Alias

Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

Description

  • Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1
  • Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils
  • Studies suggest an important role in neutrophil infiltration, recruitment and migration
  • Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function
  • The ligand for Ly-6G is currently unknown
  • Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils
  • Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity
  • Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis and depletion of neutrophils
  • Moreover, this antibody has been reported to have antagonist activity
  • (Fluorochrome-conjugated 1A8-Ly6g is recommended for use in flow cytometry.)
  • Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
  • Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C
  • The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow
  • Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes
  • Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation
  • The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.