CD81 Monoclonal Antibody (1D6-CD81), APC, eBioscience™, Invitrogen™

Description

• Description: This 1D6-CD81 monoclonal antibody reacts with human CD81, which is also known as Target of Anti-Proliferative Ab-1 (TAPA-1)
• A member of the tetraspanin superfamily of transmembrane proteins, CD81 is widely expressed on immune cells such as B, T, and NK cells, monocytes, and eosinophils
• Studies suggest that the highest expression of CD81 can be found on germinal center B cells
• This protein can also be detected on non-Hodgkin lymphomas and diffuse large B-cell lymphomas
• On B cells, CD81 exists in a complex with CD19, CD21, and Leu13
• CD81 plays a role in segregating the CD19/CD21-B cell receptor complexes to lipid rafts to activate signal transduction
• Finally, CD81 is a receptor for hepatitis C virus
• The 1D6 monoclonal antibody has been reported to induce adhesion and reduce cell proliferation
• Applications Reported: This 1D6-CD81 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1D6-CD81 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (1 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells
• CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells
• In muscles, CD81 promotes cell fusion and myotube maintenance
• CD81 has been also identified as a receptor for the hepatitis C virus
• Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains
• The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility
• CD81 is a cell surface glycoprotein that is known to complex with integrins
• CD81 appears to promote muscle cell fusion and support myotube maintenance
• CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus
• Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas
• CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.