FITC Monoclonal Antibody (NAWESLEE), APC, eBioscience™, Invitrogen™

Description

• Description: This NAWESLEE monoclonal antibody reacts to fluorescein isothyocianate (FITC), a derivative of fluorescein commonly used in flow cytometry and fluorescent microscopy
• FITC-9 can be used for the separation of cells labeled with FITC-conjugated antibodies or for staining
• Please note that in some instances the FITC signal can be quenched by the anti-FITC antibody; therefore, this phenomenon should be evaluated empirically
• Applications Reported: This NAWESLEE antibody has been reported for use in flow cytometric analysis
• Applications Tested: This NAWESLEE antibody has been tested by flow cytometric analysis of cells stained with FITC-conjugated antibody
• This can be used at less than or equal to 0.125 μg per test
• Our testing indicates that, if used at higher concentration, binding of the NAWESLEE monoclonal antibody can quench FITC fluorescence
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• FITC Monoclonal antibody specifically detects FITC in Chemical samples
• It is validated for Flow Cytometry.