Granzyme K Monoclonal Antibody (G3H69), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This G3H69 monoclonal antibody reacts with human Granzyme K
• Granzyme K is one of five granzyme serine proteases that have been identified in humans
• These proteins are expressed in the granules of NK cells and cytotoxic T cells, and are critical for the induction of target cell apoptosis through the cleavage of intracellular substrates
• Granzyme A and Granzyme K are both tryptases and appear to show overlapping, though not identical, substrate specificity
• This functional similarity is believed to account for the minimal decrease in cytotoxicity of Granzyme A-deficient CTLs
• Applications Reported: This G3H69 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This G3H69 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood monocytes
• This can be used at 5 μL (0.03 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Granzyme K (GrK) belongs to a family of trypsin-like serine proteases localised in the cytotoxic granules of activated T cells and NK cells
• It encodes a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene
• Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine
• Granzyme K triggers rapid cell death independently of caspase activation with single-stranded DNA nicks and is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.