50-112-3969
IRF8 Monoclonal Antibody (V3GYWCH), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-3969-Each-of-1 | In Stock | ₹ 37,736.00 |
50-112-3969 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,736.00
GST (18%): ₹ 6,792.48
Total Price: ₹ 44,528.48
Antigen
IRF8
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P23611, Q02556
Gene Symbols
Irf8
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
15900, 3394
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
V3GYWCH
Conjugate
PerCP-eFluor 710
Gene
Irf8
Gene Alias
AI893568; H-ICSBP; Icsbp; Icsbp1; IMD32A; IMD32B; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; Irf8; IRF-8; Myls; transcription factor ICSBP
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP)
- IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC)
- Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC
- IRF8-/- mice are deficient in both pDC and CD8a+ DC populations
- CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation
- IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice
- Applications Reported: This V3GYWCH antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This V3GYWCH antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat
- 00-5523) and protocol
- Please refer to Best Protocols: Protocol B: One-step protocol: intracellular (nuclear) proteins located under the Resources Tab online
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor
- It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes
- IRF8 plays a negative regulatory role in cells of the immune system
- It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells
- This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes
- Mutations in the gene can result in immunodeficiencey 32A and 32B.