c-MAF Monoclonal Antibody (sym0F1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-3971-Each-of-1 | In Stock | ₹ 17,088.00 |
50-112-3971 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,088.00
GST (18%): ₹ 3,075.84
Total Price: ₹ 20,163.84
Antigen
c-MAF
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
O75444, P54843
Gene Symbols
MAF
Immunogen
Full-length E. coli-produced c-Maf
Quantity
25 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG2b κ
Applications
Flow Cytometry
Clone
sym0F1
Conjugate
PerCP-eFluor 710
Gene
MAF
Gene Alias
2810401A20Rik; A230108G15Rik; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog; AW047063; AYGRP; basic domain leucine zipper transcription factor; CCA4; cMaf; C-Maf; c-Maf long form; c-maf proto-oncogene; CTRCT21; fl13d12; hMafA; LOW QUALITY PROTEIN: transcription factor Maf; LOW QUALITY PROTEIN: transcription factor MafA; maf; MAF bZIP transcription factor; MAF bZIP transcription factor A; maf protein; Maf2; MAFA; Mafa homolog; MGC71685; pancreatic beta-cell-specific transcriptional activator; Proto-oncogene c-Maf; RGD1562627; RIPE3b1; RIPE3b1 factor; T lymphocyte c-maf long form; transcription factor Maf; Transcription factor Maf-2; transcription factor MafA; transcription factor RIPE3b1; v-maf avian musculoaponeurotic fibrosarcoma oncogene A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog a (paralog a); v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A; v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian); v-maf musculoaponeurotic fibrosarcoma oncogene homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; wu:fl13d12; Z-cmaf
Host Species
Mouse
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
17132, 4094
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
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Description
- Description: The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor shown to be involved in the neural, ocular and hematopoietic systems
- In hematopoietic cells, it was first shown to be crucial for IL-4 expression in Th2 and was the first transcription factor believed to be Th subset-specific
- More recent evidence shows that specific phospho-tyrosine residues lead to upregulation of IL-4
- c-Maf has also been shown to be important to differentiation and function in both Th17 and Tfh cells
- It drives expression of IL-21 in both cell types, while promoting expression of IL-23R in Th17 and CXCR5 in Tfh as well
- Applications Reported: This sym0F1 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This sym0F1 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of Th17-polarized mouse splenocytes using the Foxp3/Transcription Factor Buffer Set and protocol
- Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins
- This can be used at 5 μL (0.0075 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- c-maf is the cellular counterpart of oncogenic v-maf that belongs to the family of basic region leucine zipper domain transcription factors
- The leucine-zipper domain is involved in the interaction with LRPICD
- There are two forms of human c-maf mRNA, c-maf-long and c-maf-short
- It is identified in the genome of the acute transforming avian retrovirus AS42
- c-maf targets are IL-4 in Th2 cells, the crystalline genes in lens fiber cells, insulin gene in islet, p53 and L7 where it exerts its transcriptional role through binding to a Maf recognition element (MARE)
- It regulates Th2 differentiation and lineage-specific hematopoiesis
- c-maf is a transcription factor for IL-10 gene expression in LPS-activated macrophages
- Chromosomal aberration involving maf is found in some forms of multiple myeloma
- It is expressed in myeloma cell lines and resting monocytes/macrophages.