Cyclin E Monoclonal Antibody (HE12), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody HE12 recognizes human Cyclin E
• Cyclins regulate the cell cycle by binding and activating cyclin-dependent kinases (CDK)
• Cyclin E regulates the transition from G1 to S phase by binding and activating CDK2
• The Cyclin E/CDK2 complex is responsible for the phosphorylation of downstream proteins that facilitate the G1 to S phase transition
• Overexpression of Cyclin E is found in several types of cancer including breast, colon, bladder, lung and skin
• In breast cancer, Cyclin E overexpression is associated with increased stage and grade as well as poor prognosis
• Applications Reported: This HE12 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and microscopy
• Applications Tested: This HE12 antibody has been tested by immunohistochemistry of FFPE human tissue using low pH antigen retreival and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle
• Cyclins function as regulators of CDK kinases
• Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event
• Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition
• This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase
• Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis
• This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB
• Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described
• Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.