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50-112-4712

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 532, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-4712-Each-of-1 In Stock ₹ 33,820.00

50-112-4712 - Each of 1

₹ 33,820.00

In Stock

Quantity

1

Base Price: ₹ 33,820.00

GST (18%): ₹ 6,087.60

Total Price: ₹ 39,907.60

Antigen

CD45

Classification

Monoclonal

Concentration

5 μL/Test

Formulation

PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P08575

Gene Symbols

PTPRC

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

5788

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry, Immunohistochemistry (Paraffin)

Clone

HI30

Conjugate

Alexa Fluor 532

Gene

PTPRC

Gene Alias

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Host Species

Mouse

Quantity

100 Tests

Primary or Secondary

Primary

Target Species

Human

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA)
  • CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets
  • The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
  • Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
  • This can be used at 5 μL (0.06 μg) per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • Alexa Fluor™ 532 is excited with the Green laser (532 nm) and emits at 561 nm
  • This cannot be used with the Yellow-Green laser (561 nm)
  • We recommend using a 560/14 band pass filter
  • Please make sure that your instrument is capable of detecting this fluorochome
  • Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
  • CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
  • CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
  • CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
  • CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.