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50-112-8601

IDO Monoclonal Antibody (mIDO-48), eFluor™ 660, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 50-112-8601-Each-of-1 In Stock ₹ 16,999.00

50-112-8601 - Each of 1

₹ 16,999.00

In Stock

Quantity

1

Base Price: ₹ 16,999.00

GST (18%): ₹ 3,059.82

Total Price: ₹ 20,058.82

Antigen

IDO

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P28776

Gene Symbols

Ido1

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

15930

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot

Clone

mIDO-48

Conjugate

eFluor 660

Gene

Ido1

Gene Alias

EC 1.13.11.52; IDO; IDO1; IDO-1; INDO; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; indoleamine 2,3-dioxygenase 1; indoleamine 23-dioxygenase; Indoleamine-2; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase

Host Species

Rat

Quantity

25 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2b κ

Description

  • Description: The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1(IDO1)
  • IDO1 is an enzyme expressed intracellularly by dendritic cells, interferon gamma activated macrophages, epithelial cells, vascular endothelium and tumor cells
  • This enzyme catalyzes tryptophan into kynurenine metabolites
  • The reduction of tryptophan and the introduction of kynurenine metabolites in the microenvironment of IDO-expressing cells suppress T cell proliferation and promote the activation and induction of regulatory T cells
  • Applications Reported: This mIDO-48 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This mIDO-48 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat
  • 88-8824) and protocol
  • Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
  • This can be used at less than or equal to 1 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin
  • By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines
  • IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs)
  • In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction
  • The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms
  • Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites
  • Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.