IgD Monoclonal Antibody (11-26c (11-26)), Biotin, eBioscience™, Invitrogen™

Description

• Description: The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD
• It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM
• IgD is expressed by peripheral mature B cells
• 11-26c does not activate B cells
• Applications Reported: The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells
• Applications Tested: The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.