50-112-8830
GARP Monoclonal Antibody (G14D9), eFluor™ 660, eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-8830-Each-of-1 | In Stock | ₹ 38,626.00 |
50-112-8830 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,626.00
GST (18%): ₹ 6,952.68
Total Price: ₹ 45,578.68
Antigen
GARP
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
Q14392
Gene Symbols
LRRC32
Immunogen
A mixture of mGARP-Fc and hGARP 21H-F1 & -F2
Quantity
100 Tests
Primary or Secondary
Primary
Target Species
Human
Product Type
Antibody
Isotype
IgG2a κ
Applications
Flow Cytometry
Clone
G14D9
Conjugate
eFluor 660
Gene
LRRC32
Gene Alias
AI426318; D11S833E; D11S833Eh; D7H11S833E; EG434215; Garp; garpin; Glycoprotein A repetitions predominant; leucine rich repeat containing 32; leucine-rich repeat-containing protein 32; Lrrc32; Transforming growth factor beta activator LRRC32
Host Species
Rat
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
2615
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
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Description
- Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predomit (GARP, also known as LRRC32 or Garpin)
- GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface
- Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas
- Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells
- The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta
- Applications Reported: This G14D9 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This G14D9 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
- This can be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- eFluor™ 660 is a replacement for Alexa Fluor™ 647
- eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
- Please make sure that your instrument is capable of detecting this fluorochome
- GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region
- GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation
- Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.