Phospho-p38 MAPK (Thr180, Tyr182) Monoclonal Antibody (4NIT4KK), PE, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-9313-Each-of-1 | In Stock | ₹ 14,596.00 |
50-112-9313 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,596.00
GST (18%): ₹ 2,627.28
Total Price: ₹ 17,223.28
Antigen
Phospho-p38 MAPK (Thr180, Tyr182)
Classification
Monoclonal
Concentration
5 μL/Test
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.
P47811, Q16539
Gene Symbols
MAPK14
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
1432, 26416
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
4NIT4KK
Conjugate
PE
Gene
MAPK14
Gene Alias
Crk1; CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug binding protein 1; cytokine suppressive anti-inflammatory drug-binding protein; cytokine-supressive anti-inflammatory drug binding protein; Exip; Hog; MAP kinase 11; MAP kinase 13; MAP kinase 14; MAP kinase 2; MAP kinase Mxi2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAPK 11; MAPK 13; MAPK 14; Mapk11; Mapk13; MAPK-13; Mapk14; MAX-interacting protein 2; mitogen activated protein kinase 11; mitogen activated protein kinase 13; mitogen activated protein kinase 14; mitogen-activated protein kinase 11; Mitogen-activated protein kinase 13; mitogen-activated protein kinase 14; mitogen-activated protein kinase 14A; mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; mitogen-activated protein kinase p38 delta; mitogen-activated protein kinase p38-2; MXI2; p38; p38 alpha; p38 delta MAP kinase; p38 MAP kinase; p38 MAP kinase alpha; p38 MAPK; p38 mitogen activated protein kinase; p38-2; p38a; p38ALPHA; p38-alpha; p38alpha Exip; P38b; p38Beta; p38beta2; p38delta; p38Hog; p38MAPK; Prkm11; PRKM13; PRKM14; Prkm15; protein kinase, mitogen activated kinase, 11, p38beta; reactive kinase; RK; SAPK/Erk/kinase 4; SAPK2; SAPK2A; SAPK2B; SAPK4; Serk4; Stress-activated protein kinase 2a; Stress-activated protein kinase 2b; Stress-activated protein kinase 4; stress-activated protein kinase-2; stress-activated protein kinase-2b; tRNA synthetase cofactor p38
Host Species
Mouse
Quantity
25 Tests
Primary or Secondary
Primary
Target Species
Human, Mouse
Product Type
Antibody
Isotype
IgG2b κ
Related Products
Description
- Description: This 4NIT4KK monoclonal antibody recognizes human and mouse p38 mitogen-activated protein kinase (MAPK) when phosphorylated on T180/Y182
- p38 MAPK belongs to a family of conserved serine/threonine protein kinases that are phosphorylated and activated in response to numerous stress stimuli including TLR ligands (such as LPS), osmotic shock, heat shock, UV irradiation, and inflammatory cytokines
- There are four p38 MAPK members that include p38 alpha, p38 beta, p38 gamma, and p38 delta
- The primary activators of p38 MAPK are MKK3/4 and MKK6
- Several downstream targets of p38 MAPK have been identified including MK2/3, p53, ATF-2, and ETS1
- p38 MAPK can be negatively regulated by the chemical inhibitor SB203580
- Specificity of this 4NIT4KK clone was determined by ELISA, flow cytometry, and western blotting
- Applications Reported: This 4NIT4KK antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This 4NIT4KK antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.06 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- The p38 MAPK cascade regulates a variety of cellular responses to stress, inflammation, and other signals
- p38 MAPK is relatively inactive in the nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motifs
- There are four isoforms of p38 MAPK, which differ in their tissue expression and affinity for upstream activators and downstream effectors
- When cells are exposed to tumor necrosis factor, interleukin-1, heat shock, or other activating stimuli, activation of MAPK kinase-3 occurs by phosphorylation
- Activated MAPK kinase-3/6 phosphorylate each residue of Thr180 and Tyr182 in p38 MAPK
- Phospho-p38 MAPK activates ATF-2, CHOP-1, MEF-2 and other transcription factors through phosphorylation.