GL7 Monoclonal Antibody (GL-7 (GL7)), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi
• There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6
• GL7 is commonly used as a marker for mouse germinal center B cells
• The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac
• This moiety is recognized by CD22
• Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi
• There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6
• GL7 is commonly used as a marker for mouse germinal center B cells
• The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac
• This moiety is recognized by CD22.