Ki-67 Monoclonal Antibody (20Raj1), eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-131-21-Each-of-1 | In Stock | ₹ 37,380.00 |
50-131-21 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,380.00
GST (18%): ₹ 6,728.40
Total Price: ₹ 44,108.40
Antigen
Ki-67
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P46013
Gene Symbols
Mki67
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
100686578, 4288
Content And Storage
4° C
Form
Liquid
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin)
Clone
20Raj1
Conjugate
Unconjugated
Gene
Mki67
Gene Alias
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Canine, Human
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein
- Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells
- Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0)
- During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3)
- In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells
- This monoclonal antibody 20Raj1 recognizes canine (dog) Ki-67
- Applications Reported: This 20Raj1 antibody has been reported for use in immunocytochemistry and immunohistochemistry on formalin-fixed paraffin embedded tissues
- Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on paraformaldehyde fixed and permeabilized MCF7, MDCK, and MDA-MB-175 cell lines
- This 20Raj1 antibody has been tested by immunohistochemistry on FFPE human skin using citrate buffer antigen retrieval
- For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 0.5-1 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases
- The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus
- Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase
- Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis
- Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms
- In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index
- The correlation between low Ki-67 index and histologically low-grade tumors is strong
- Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.