Embigin Monoclonal Antibody (G7.43.1), eBioscience™, Invitrogen™

Description

• Description: This G7.43.1 monoclonal antibody reacts with mouse embigin (gp70), a single transmembrane glycoprotein that is a member of the Ig superfamily similar to basigin and EMMPRIN
• It contains two Ig domains thought to be involved in cellular adhesion and tissue remodeling
• Expression of embigin is high before day 10 in fetal development
• In the adult it is expressed on myeloid cells and T cells but not B cells
• During hematopoeisis, embigin is expressed on HSCs and CLPs (common lymphoid progenitors) but absent on pro- and pre-B cells
• In recent studies Pax 5 has been shown to repress embigin expression
• Applications Reported: This G7.43.1 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections
• Applications Tested: This G7.43.1 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains
• In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis
• A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues
• Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion
• Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.