Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells
• Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells
• Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells
• Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers
• Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP
• Applications Reported: This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry
• Applications Tested: This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low or high pH antigen retrieval
• This can be used at less than or equal to 10 μg/mL
• Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
• Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
• Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
• Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
• Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.