50-132-77
Ly-49H Monoclonal Antibody (3D10), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-132-77-Each-of-1 | In Stock | ₹ 14,596.00 |
50-132-77 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 14,596.00
GST (18%): ₹ 2,627.28
Total Price: ₹ 17,223.28
Antigen
Ly-49H
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
Q60682
Gene Symbols
Klra8
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16639
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Clone
3D10
Conjugate
Unconjugated
Gene
Klra8
Gene Alias
Cmv1; Cmv-1; cytomegalovirus resistance 1; killer cell lectin-like receptor 8; killer cell lectin-like receptor, subfamily A, member 8; Klra8; Ly49H; Ly-49h; Ly49-h; Ly49u<129>; Lymphocyte antigen 49h; T-cell surface glycoprotein Ly-49H
Host Species
Mouse
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The monoclonal antibody 3D10 recognizes Ly-49H, a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells
- Ly-49H, like Ly-49D, lacks the characteristic ITIM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors
- Ly-49H is expressed in a subset of NK cells but not NKT (NK1.1CD3+) cells
- Natural killer cells can express several Ly-49 proteins
- It has been shown that there is preferential staining of Ly-49H with Ly-49D (cat 12-5783)
- Expression has been confirmed on C57BL/6 and NWNA but not Balb/c or DBA/2 mice
- Other mice strains have not been tested
- No crossreactivity to Ly-49A, C, D or G2 has been observed
- Addition of monoclonal antibody 3D10 to cell cultures induces lysis of target cells
- Applications Reported: This 3D10 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation
- Applications Tested: This 3D10 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Ly49H is a member of the C-type lectin Ly-49 multigene family of receptors found on natural killer cells
- Ly49H, like Ly49D, lacks the characteristic ITAM (immunoreceptor tyrosine-based inhibitory motif) and contains an arginine in the transmembrane domain implying these molecules act as activation receptors
- Ly49H is expressed in a subset of NK cells but not NKT (NK1.1/CD3+) cells
- Natural killer cells can express several Ly49 proteins.