Integrin alpha 4 beta 7 (LPAM-1) Monoclonal Antibody (DATK32 (DATK-32)), eBioscience™, Invitrogen™
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-132-79-Each-of-1 | In Stock | ₹ 10,235.00 |
50-132-79 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 10,235.00
GST (18%): ₹ 1,842.30
Total Price: ₹ 12,077.30
Antigen
Integrin alpha 4 beta 7 (LPAM-1)
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P26011, Q00651
Gene Symbols
Itga4, Itgb7
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16401, 16421
Content And Storage
4° C
Form
Liquid
Applications
Flow Cytometry, Functional Assay
Clone
DATK32 (DATK-32)
Conjugate
Unconjugated
Gene
Itgb7
Gene Alias
CD49 antigen-like family member D; CD49D; integrin alpha 4; integrin alpha-4; integrin alpha4B; Integrin alpha-IV; integrin beta 7; integrin beta-7; Integrin beta-P; Itga4; Itga4B; Itgb7; LPAM subunit alpha; Ly69; lymphocyte Peyer patch adhesion molecules subunit alpha; M290 IEL antigen; VLA; VLA 4; VLA-4 receptor, alpha 4 subunit; VLA4 subunit alpha; VLA-4 subunit alpha
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG2a κ
Related Products
Description
- Description: The DATK32 monoclonal antibody binds to a combinatorial epitope of mouse LPAM-1 (alpha4beta7), which is a heterodimer of the the 154 kDa alpha4 subunit and the 130 kDa beta7 subunit
- In mouse, the beta7 subunit is found on the majority of mature lymphocytes and a small population of thymocytes and bone marrow cells
- LPAM-1 is an integrin involved in the transendothelial migration of lymphocytes across high endothelial venules (HEV), and is know to bind several ligands including MAdCAM-1, VCAM-1 and fibronectin
- Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block LPAM-1-mediated cell adhesion
- Applications Reported: This DATK32 (DATK-32) antibody has been reported for use in flow cytometric analysis
- (Please use Functional Grade purified DATK32 (DATK-32), cat
- 16-5887, in functional assays.)
- Applications Tested: This DATK32 (DATK-32) antibody has been tested tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes
- It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes
- Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324)
- Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.