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50-133-60

CD272 (BTLA) Monoclonal Antibody (6G3), eBioscience™, Invitrogen™

Manufacturer: Invitrogen

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Pack Size SKU Availability Price
Each of 1 50-133-60-Each-of-1 In Stock ₹ 29,548.00

50-133-60 - Each of 1

₹ 29,548.00

In Stock

Quantity

1

Base Price: ₹ 29,548.00

GST (18%): ₹ 5,318.64

Total Price: ₹ 34,866.64

Antigen

CD272 (BTLA)

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

Q7TSA3

Gene Symbols

BTLA

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

208154

Content And Storage

4° C

Form

Liquid

Applications

Flow Cytometry

Clone

6G3

Conjugate

Unconjugated

Gene

BTLA

Gene Alias

A630002H24; B and T lymphocyte associated; B and T lymphocyte associated protein; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; BTLA1; CD272; CD272 antigen; FLJ16065; MGC129743

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG1 κ

Description

  • Description: The 3F9.D12 antibody reacts with mouse BTLA, B and T lymphocyte attenuator from both BALB/c and C57Bl/6 strains
  • BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in thymus and mature, but not immature bone marrow-derived dendritic cells
  • BTLA is implicated as a negative regulator of the activation and/or function of various hemopoietic cell types
  • It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction
  • Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4+ and CD8+ single-positive (SP) thymocytes (Hurchla et al)
  • However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes
  • It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells
  • Applications Reported: This 6G3 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 6G3 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This can be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily
  • BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses
  • BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation
  • It remains expressed on Th1 cells, but not Th2 cells
  • BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM)
  • This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.