TNF alpha Monoclonal Antibody (TN3-19.12), eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-137-02-Each-of-1 | In Stock | ₹ 26,077.00 |
50-137-02 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 26,077.00
GST (18%): ₹ 4,693.86
Total Price: ₹ 30,770.86
Antigen
TNF alpha
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P06804, P16599
Gene Symbols
Tnf
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
21926, 24835
Content And Storage
4° C
Form
Liquid
Applications
ELISA, Flow Cytometry, Functional Assay, Neutralization
Clone
TN3-19.12
Conjugate
Unconjugated
Gene
Tnf
Gene Alias
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Host Species
Armenian Hamster
Quantity
500 μg
Primary or Secondary
Primary
Target Species
Mouse, Rat
Product Type
Antibody
Isotype
IgG
Related Products
Description
- Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha
- The TN3-19.12 antibody is a neutralizing antibody
- Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells
- A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule
- TNF alpha is cytolytic and plays an important role in immune regulation
- Dimers and trimers of TNF alpha have been observed
- Applications Reported: The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, ELISPOT, IVCCA, and ELISA
- Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry
- Functional Grade purified TN3-19.12, cat
- 16-7423, is recommended for use in functional assays
- For ELISPOT capture, the alternative clone, 1F3F3D4 (cat No
- 16-7325), is recommended
- Applications Tested: The TN3-19.12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse or rat TNF alpha in combination with the biotinylated anti-mouse/rat TNF alpha polyclonal antibody (13-7341) for detection and recombit mouse TNF alpha (14-8321) or rat TNF alpha as the standard
- Product 14-8320 is a bioactive protein, but is not recommended for rat TNF alpha ELISA.] A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL
- TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
- This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
- TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
- TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
- Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
- Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
- TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
- This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.